Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle ExplantsMegan Vaughan 1, Katja A. Lamia 1
1Scripps Research, Department of Molecular Medicine
Myoblasts are proliferating precursor cells that differentiate to form polynucleated myotubes and eventually skeletal muscle myofibers. Here, we present a protocol for efficient isolation and culture of primary myoblasts from young adult mouse skeletal muscles. The method enables molecular, genetic, and metabolic studies of muscle cells in culture.
