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Fralin Biomedical Research Institute at Virginia Tech Carilion

2 ARTICLES PUBLISHED IN JoVE

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Behavior

Inducing Post-Traumatic Epilepsy in a Mouse Model of Repetitive Diffuse Traumatic Brain Injury
Oleksii Shandra 1, Stefanie Robel 1,2,3
1Fralin Biomedical Research Institute at Virginia Tech Carilion, 2School of Neuroscience, Virginia Tech, 3Department of Biological Sciences, Virginia Tech

This systematic protocol describes a new animal model of post-traumatic epilepsy after repetitive mild traumatic brain injury. The first part details steps for traumatic brain injury induction using a modified weight drop model. The second part provides instructions on the surgical approach for single- and multi-channel electroencephalographic data acquisition systems.

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A Photopolymerizable Hyaluronic Acid-Collagen Model of the Invasive Glioma Microenvironment with Interstitial Flow

A Photopolymerizable Hyaluronic Acid-Collagen Model of the Invasive Glioma Microenvironment with Interstitial Flow
Samantha Howerton 1,2, Yanping Liang 1, Jennifer Hammel 1,3, Benjamin Purow 4, Jennifer Munson 1,3
1Fralin Biomedical Research Institute at Virginia Tech Carilion, 2Translational Biology, Medicine, and Health Graduate Program, Virginia Tech, 3Department of Biomedical Engineering & Mechanics, Virginia Tech, 4Department of Neurology, University of Virginia School of Medicine

We present a method for replicating the glioma tumor microenvironment at the invasive front that incorporates interstitial fluid flow. This model is a hyaluronan-collagen I hydrogel in a tissue culture insert where a fluid pressure head can be applied. Invasion can be quantified, and cells can be isolated or lysed.

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