Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness AnalysisMoreno Zamai 1, Antonio Trullo 2, Elvira Arza 1, Ugo Cavallaro 3, Valeria R. Caiolfa 1,2
1Unit of Microscopy and Dynamic Imaging, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain, 2Centro di Imaging Sperimentale, Ospedale San Raffaele, Milan, Italy, 3Unit of Gynecological Oncology Research, European Institute of Oncology IRCCS, Milan, Italy
We describe an imaging approach for the determination of the average oligomeric state of mEGFP-tagged-receptor oligomers induced by ligand binding in the plasma membrane of living cells. The protocol is based on Total Internal Reflection Fluorescence (TIRF) microscopy combined with Number and Brightness (N&B) analysis.
