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Long Island University

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
Yi Xu *1, Samiksha Wasnik *1, David J. Baylink 1, Edmundo Carreon Berumen 1, Xiaolei Tang 1
1Department of Medicine, Division of Regenerative Medicine, Loma Linda University

Qa-1 (HLA-E in human) belongs to a group of non-classical major histocompatibility complex 1b molecules. Immunization with Qa-1-binding epitopes has been shown to augment tissue-specific immune regulation and ameliorate several autoimmune diseases. Herein we describe an overlapping peptide library strategy for the identification of Qa-1 epitopes in a protein.

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Immunology and Infection

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
Hongzheng Bi 1,2, Samiksha Wasnik 1, David J. Baylink 1, Chenfan Liu 1,3,4, Xiaolei Tang 1,3
1Division of Regenerative Medicine, Department of Medicine, Loma Linda University, 2Zhengzhou University, 3Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, Long Island University, 4Jinan Infectious Disease Hospital, Shandong University

Here we present a protocol for in vivo augmentation of gut-homing regulatory T cell induction. In this protocol, dendritic cells are engineered to locally produce high concentrations of the active vitamin D (1,25-dihydroxyvitamin D or 1,25[OH]2D) and the active vitamin A (retinoic acid or RA) de novo.

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