Electrochemotherapy in 3D Ocular Melanoma Spheroids using a Customized ElectrodeMiltiadis Fiorentzis 1,2, Arne Viestenz 1, Berthold Seitz 3, Sarah E Coupland 4, Joana Heinzelmann 1
1Department of Ophthalmology, University Hospital Halle (Saale), Martin-Luther University Halle-Wittenberg, 2Department of Ophthalmology, University Hospital Essen, Duisburg Essen University, 3Department of Ophthalmology, Saarland University Medical Center, 4Liverpool Ocular Oncology Research Group, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool
Here, we present a protocol for the development of 3D conjunctival and uveal melanoma spheroids and the use of hand-held customized electrodes for in vitro electrochemotherapy of 3D spheroids in a culture well. This offers new perspectives in the use of electrochemotherapy in ocular melanoma treatment.
