Cofactor Genomics

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Dawn M. Johnson¹, Natalie A. LaFranzo¹, Joshua A. Maurer¹

¹Department of Chemistry, Washington University in St. Louis

Self-assembled monolayers (SAMs) formed from long chain alkane thiols on gold provide well-defined substrates for the formation of protein patterns and cell confinement. Microcontact printing of hexadecanethiol using a polydimethylsiloxane (PDMS) stamp followed by backfilling with a glycol-terminated alkane thiol monomer produces a pattern where protein and cells adsorb only to the stamped hexadecanethiol region.

Immunology and Infection

Predictive Immune Modeling of Solid Tumors

Natalie A. LaFranzo¹, Kevin C. Flanagan¹, Danielle Quintanilha¹

¹Cofactor Genomics

The use of an RNA-based approach to determine quantitative immune profiles of solid tumor tissues and leverage clinical cohorts for immune-oncology biomarker discovery is described through a molecular and informatics protocols.