JoVE | Peer Reviewed Scientific Video Journal

Genetics

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Tanita Wein^*1, Fenna T. Stücker^*1, Nils F. Hülter¹, Tal Dagan¹

¹Institute of Microbiology, Kiel University

Our experimental approach provides a strategy to follow plasmid abundance and antibiotic resistance over time in bacterial populations.

Engineering

Treating Surfaces with a Cold Atmospheric Pressure Plasma using the COST-Jet

Judith Golda¹, Kerstin Sgonina¹, Julian Held², Jan Benedikt¹, Volker Schulz-von der Gathen²

¹Experimental Plasma Physics, Kiel University, ²Experimental Physics II, Ruhr-University Bochum

This protocol is presented to characterize the setup, handling, and application of the COST-Jet for the treatment of diverse surfaces such as solids and liquids.

Chemistry

Discovery and Synthesis Optimization of Isoreticular Al(III) Phosphonate-Based Metal-Organic Framework Compounds Using High-Throughput Methods

Marvin Radke¹, Ralf Suren¹, Norbert Stock¹

¹Department of Inorganic Chemistry, Kiel University

The targeted synthesis of new metal-organic frameworks (MOFs) is difficult, and their discovery depends on the knowledge and creativity of the chemist. High-throughput methods allow complex synthetic parameter fields to be explored quickly and efficiently, accelerating the process of finding crystalline compounds and identifying synthetic and structural trends.

Environment

JenaTron - An Experimental Approach to Study the Effects of Plant History and Soil History on Grassland Ecosystem Functioning

Anna-Maria Madaj^1,2, Yuanyuan Huang^1,2, Anne Ebeling³, Lisa Ertel^1,2, Alban Gebler^1,2, Gerd Gleixner^1,4,5, Jes Hines^1,2, Christiane Roscher^1,6, Alexandra Weigelt^1,2, Cynthia Albracht^7,8,9, Angelos Amyntas^1,10, Leonardo Bassi^1,2, Ana Elizabeth Bonato Asato^1,2, Michael Bonkowski^11,12, Maximilian Bröcher³, François Buscot^1,8, Francesca De Giorgi^1,6, Yuri Pinheiro Alves de Souza^13,14, Van Cong Doan^1,10, Walter Durka^1,15, Anna Heintz-Buschart⁷, Justus Hennecke^1,2, Markus Lange⁴, Pamela Medina-van Berkum¹⁶, Sebastian Tobias Meyer¹⁴, Sören Krawczyk^1,2, Akanksha Rai⁴, Thomas Reitz^1,8, Christian Ristok^1,2, Stefan Scheu^17,18, Michael Schloter^13,14, Stefanie Schulz¹³, Marcel Dominik Solbach¹¹, Sybille Barbara Unsicker^16,19, Nico Eisenhauer^1,2

¹German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, ²Institute of Biology, Leipzig University, ³Institute of Ecology and Evolution, Friedrich Schiller University Jena, ⁴Max Planck Institute for Biogeochemistry, ⁵Institute for Geosciences, Friedrich Schiller University Jena, ⁶Department Physiological Diversity, Helmholtz Centre for Environmental Research (UFZ), ⁷Swammerdam Institute for Life Sciences, University of Amsterdam, ⁸Department Soil Ecology, Helmholtz Centre for Environmental Research (UFZ), ⁹Institute Biosafety in Plant Biotechnology, Julius Kühn-Institute, ¹⁰Institute of Biodiversity, Friedrich Schiller University Jena, ¹¹Institute of Zoology, University of Cologne, ¹²Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, ¹³Research Unit Comparative Microbiome Analysis, Helmholtz Zentrum Munich, ¹⁴School of Life Science, Technical University Munich - TUM, ¹⁵Department of Community Ecology, Helmholtz Centre for Environmental Research (UFZ), ¹⁶Department of Biochemistry, Max-Planck Institute for Chemical Ecology, ¹⁷JFB Institute of Zoology and Anthropology, University of Göttingen, ¹⁸Centre of Biodiversity and Sustainable Land Use, University of Göttingen, ¹⁹Botanical Institute, Plant-Environment-Interactions Group, Kiel University

Here we present a study established in the iDiv Ecotron, terrestrial mesocosm chambers with controlled environmental conditions above- and below-ground, to study the independent and interactive effects of plant history and soil history on ecosystem functioning of grassland communities along a plant diversity gradient.

Kiel University

4 ARTICLES PUBLISHED IN JoVE

Genetics

Engineering

Chemistry

Environment