Stony Brook Cancer Center, Stony Brook, NY

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

David C. Montrose^1,2, Erin M. McNally³, Erika Sue³, Andrew J. Dannenberg³

¹Department of Pathology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, ²Stony Brook Cancer Center, Stony Brook, NY, ³Department of Medicine, Weill Cornell Medicine, New York, NY

Here, we provide a detailed description of the procedure to induce colonoscopic-guided pinch biopsies in mice and track wound closure in real time. Additionally, methods for the preparation of tissues for histological, immunohistochemical and molecular analyses of the wound bed are provided.