King Mongkut's University of Technology North Bangkok (KMUTNB)

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

Theerawut Phusantisampan¹, Pattarasuda Rawiwan^2,3, Sri Rajiv Kumar Roy², Malinee Sriariyanun⁴, Win Surachetpong^2,3

¹Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), ²Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, ³Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, ⁴Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries.