Non-Radioactive In Vitro Cardiac Myosin Light Chain Kinase AssaysKenta Kamikubo *1, Osamu Tsukamoto *1, Yuki Uyama-Saito *1, Ryohei Oya 1, Tomoya Tsubota 1, Noboru Fujino 3, Yoshihiro Asano 2, Hisakazu Kato 1, Ken Matsuoka 1, Seiji Takashima 1
1Department of Medical Biochemistry, Osaka University Graduate School of Medicine/Frontier Biosciences, 2Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine/Frontier Biosciences, 3Department of Cardiovascular and internal medicine, Kanazawa University Graduate School of Medicine
This study describes protocols for nonradiometric methods, a bioluminescent ADP detection assay and a phosphate-affinity SDS-PAGE, to determine the kinase activity of cardiac myosin light chain kinase (cMLCK) and the phosphorylation level of its substrate, myosin regulatory light chain (MLC2v).
