Jouf University

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Hamad H. Alanazi^1,2, Li She^1,3, Xiao-Dong Li¹

¹Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, University of Texas Health San Antonio, ²Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, ³Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University

Environmental allergens such as house dust mites (HDM) often contain microbial substances that activate innate immune responses to regulate allergic inflammation. The protocol presented here demonstrates the identification of dsRNA species in HDM allergens and characterization of their immunogenic activities in modulating eosinophilic lung inflammation.

Neuroscience

Enhancing Electrode Location Assessment in Cochlear Implantation via Computed Tomography Image Fusion

Asma Alahmadi^*1, Nezar Hamed^*1, Yassin Abdelsamad², Tahir Sharif², Afrah Alshalan³, Farid Alzhrani¹

¹King Abdullah Ear Specialist Center (KAESC), College of Medicine, King Saud University Medical City (KSUMC), King Saud University, ²Research Department, MED-EL GmbH, ³Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Jouf University

This protocol demonstrates a method for fusing pre-operative and post-operative computed tomography images for cochlear implant recipients. The approach may enhance the measurement accuracy of insertion depth and the center frequency of electrode array contacts. Additionally, this method has potential applications in anatomy-based fitting, an emerging area within the cochlear implant field.

Immunology and Infection

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro

Muhanna Alshehri^1,2, Saeed Alqahtani^1,2,3, Pranitha Murali^2,4,5, Christopher Delaney^1,2, William Johnston^2,4,5, Jason L. Brown^1,2

¹Oral Sciences Research Group, Glasgow Dental School, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, ²Glasgow Biofilm Research Network, ³Department of Restorative Dental Sciences, College of Dentistry, Jouf University, ⁴Department of Biological and Biomedical Sciences, Glasgow Caledonian University, ⁵Safeguarding Health through Infection Prevention Research Group, Glasgow Caledonian University

The present protocol outlines the application of organotypic tissue models for investigating host-pathogen interactions in vitro. Specifically, the model system described uses a multi-layered oral epithelium exposed to microorganisms associated with biofilms (dental plaque) within the context of oral health versus disease.