University of Nottingham Biodiscovery Institute

1 ARTICLES PUBLISHED IN JoVE

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Paulina M. Durczak^*1, Kathryn L. Fair^*1, Nicholas Jinks¹, Sara Cuevas Ocaña¹, Carlos B. Sainz Zuñiga¹, Nicholas R.F. Hannan¹

¹Biodiscovery Institute, Translational Medical Sciences, School of Medicine, University of Nottingham

This protocol allows for the differentiation of human pluripotent cells into intestinal organoids. The protocol mimics normal human development by differentiating cells into a population of definitive endoderm, hindgut endoderm and then intestinal epithelium. This makes the protocol suitable for studying both intestinal development as well as disease modelling applications.