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Leipzig University

3 ARTICLES PUBLISHED IN JoVE

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Biology

MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T
Remco van Schadewijk 1, Julia R. Krug 2,3,4, Andrew Webb 5, Henk Van As 2,4, Aldrik H. Velders 3,4, Huub J. M. de Groot 1, A. Alia 1,6
1Solid-state NMR, Leiden Institute of Chemistry, Faculty of Science, Leiden University, 2Laboratory of Biophysics, Wageningen University & Research, 3Laboratory of BioNanoTechnology, Wageningen University & Research, 4MAGNEtic resonance Facility, Wageningen University & Research, 5C.J. Gorter Center for High Field MRI, Radiology department, Leiden University Medical Centre, Leiden University, 6Institute for Medical Physics and Biophysics, Leipzig University

A protocol to study biological tissue at high spatial resolution using ultra-high field magnetic resonance microscopy (MRM) using microcoils is presented. Step-by-step instructions are provided for characterizing the microcoils. Finally, optimization of imaging is demonstrated on plant roots.

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Biochemistry

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
Raphaël de Wijn 1,4, Kévin Rollet 1,2, Vincent Olieric 3, Oliver Hennig 2, Nicola Thome 1, Camille Noûs 1, Caroline Paulus 1, Bernard Lorber 1, Heike Betat 2, Mario Mörl 2, Claude Sauter 1
1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS, Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry, Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH

A versatile microfluidic device is described that enables the crystallization of an enzyme using the counter-diffusion method, the introduction of a substrate in the crystals by soaking, and the 3D structure determination of the enzyme:substrate complex by a serial analysis of crystals inside the chip at room temperature.

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Neuroscience

Neuronavigated Focalized Transcranial Direct Current Stimulation Administered During Functional Magnetic Resonance Imaging
Filip Niemann *1, Alireza Shahbabaie *1, Sven Paßmann 1, Steffen Riemann 1, Robert Malinowski 1, Harund Kocataş 1, Leonardo M. Caisachana Guevara 1,2, Mohamed Abdelmotaleb 1, Daria Antonenko 1, Felix Blankenburg 3,4, Rico Fischer 2, Gesa Hartwigsen 5,6, Shu-Chen Li 7,8, Michael A. Nitsche 9,10,11, Axel Thielscher 12,13, Dagmar Timmann 14, Anna Fromm 1, Dayana Hayek 1, Ann-Kathrin Hubert 1, Andrew K. Martin 15,16, Alexander Hunold 17, Agnes Flöel *1,18, Marcus Meinzer *1
1Department of Neurology, University Medicine Greifswald, 2Department of Psychology, University Medicine Greifswald, 3Berlin School of Mind and Brain, Humboldt Universität zu Berlin, 4Neurocomputation and Neuroimaging Unit, Freie Universität Berlin, 5Wilhelm-Wundt-Institute for Psychology, Cognitive and Biological Psychology, Leipzig University, 6Lise Meitner Research Group Cognition and Plasticity, Max Planck Institute for Human Cognitive and Brain Sciences, 7Lifespan Developmental Neuroscience, Faculty of Psychology, Technische Universität Dresden, 8Centre for Tactile Internet with Human-in-the-Loop, Technische Universität Dresden, 9Department of Psychology and Neurosciences, Leibniz Research Centre for Working Environment and Human Factors, 10German Centre for Mental Health (DZPG), 11Clinic of Psychiatry and Psychotherapy, Protestant Hospital of Bethel Foundation, University Hospital OWL, Bielefeld University, 12Danish Research Centre for Magnetic Resonance, Centre for Functional and Diagnostic Imaging and Research, Copenhagen University Hospital Amager and Hvidovre, 13Department of Health Technology, Technical University of Denmark, 14Department of Neurology and Center for Translational Neuro- and Behavioral Sciences (C-TNBS), Essen University Hospital, 15Department of Psychology, University of Kent, 16Kent Medway Medical School, 17Institute of Biomedical Engineering and Informatics, Technische Universität Ilmenau, 18German Center for Neurodegenerative Diseases (DZNE Site Greifswald)

This protocol describes the method of neuronavigated electrode placement for focal, transcranial direct current stimulation (tDCS) administered during functional magnetic resonance imaging (fMRI).

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