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Leipzig University

2 ARTICLES PUBLISHED IN JoVE

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Biology

MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T
Remco van Schadewijk 1, Julia R. Krug 2,3,4, Andrew Webb 5, Henk Van As 2,4, Aldrik H. Velders 3,4, Huub J. M. de Groot 1, A. Alia 1,6
1Solid-state NMR, Leiden Institute of Chemistry, Faculty of Science, Leiden University, 2Laboratory of Biophysics, Wageningen University & Research, 3Laboratory of BioNanoTechnology, Wageningen University & Research, 4MAGNEtic resonance Facility, Wageningen University & Research, 5C.J. Gorter Center for High Field MRI, Radiology department, Leiden University Medical Centre, Leiden University, 6Institute for Medical Physics and Biophysics, Leipzig University

A protocol to study biological tissue at high spatial resolution using ultra-high field magnetic resonance microscopy (MRM) using microcoils is presented. Step-by-step instructions are provided for characterizing the microcoils. Finally, optimization of imaging is demonstrated on plant roots.

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Biochemistry

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
Raphaël de Wijn 1,4, Kévin Rollet 1,2, Vincent Olieric 3, Oliver Hennig 2, Nicola Thome 1, Camille Noûs 1, Caroline Paulus 1, Bernard Lorber 1, Heike Betat 2, Mario Mörl 2, Claude Sauter 1
1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS, Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry, Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH

A versatile microfluidic device is described that enables the crystallization of an enzyme using the counter-diffusion method, the introduction of a substrate in the crystals by soaking, and the 3D structure determination of the enzyme:substrate complex by a serial analysis of crystals inside the chip at room temperature.

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