University of Toronto - Mississauga

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Dissection, Immunohistochemistry and Mounting of Larval and Adult Drosophila Brains for Optic Lobe Visualization

Urfa Arain¹, Priscilla Valentino¹, Ishrat Maliha Islam¹, Ted Erclik¹

¹Departments of Biology and Cell & Systems Biology, University of Toronto - Mississauga

This protocol describes three steps to prepare larval and adult Drosophila optic lobes for imaging: 1) brain dissections, 2) immunohistochemistry and 3) mounting. Emphasis is placed on step 3, as distinct mounting orientations are required to visualize specific optic lobe structures.