Dissection and Isolation of Murine Glia from Multiple Central Nervous System RegionsMaksim Sinyuk 1, Jessica L. Williams 1,2
1Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, 2Brain Health Research Institute, Kent State University
Here we present a protocol for in vitro isolation of multiple glial cell populations from a mouse CNS. This method allows for the segregation of regional microglia, oligodendrocyte precursor cells, and astrocytes to study the phenotypes of each in a variety of culture systems.