Lerner Research Institute, Cleveland Clinic Foundation

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

¹Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, ²Brain Health Research Institute, Kent State University

Here we present a protocol for in vitro isolation of multiple glial cell populations from a mouse CNS. This method allows for the segregation of regional microglia, oligodendrocyte precursor cells, and astrocytes to study the phenotypes of each in a variety of culture systems.