Creating Matched In vivo/In vitro Patient-Derived Model Pairs of PDX and PDX-Derived Organoids for Cancer Pharmacology ResearchXiaoxi Xu 1, Limei Shang 1, Philip Wang 2, Jun Zhou 2, Xuesong Ouyang 2, Meiling Zheng 1, Binchen Mao 2, Likun Zhang 2, Bonnie Chen 1, Jingjing Wang 2, Jing Chen 3, Wubin Qian 2, Sheng Guo 2, Yujun Huang 2, Qi-Xiang Li 3
1Crown Bioscience Inc., Changping District, Beijing, China, 2Crown Bioscience Inc., Taicang, Jiangsu, China, 3Crown Bioscience Inc., San Diego, CA, USA
A method is described to create organoids using patient-derived xenografts (PDX) for in vitro screening, resulting in matched pairs of in vivo/in vitro models. PDX tumors were harvested/processed into small pieces mechanically or enzymatically, followed by the Clevers’ method to grow tumor organoids that were passaged, cryopreserved and characterized against the original PDX.
