National Centre for Nuclear Research, Sołtana 7, 05-400 Otwock, Poland

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

Kamila Maliszewska-Olejniczak¹, Agnieszka Dróżdż¹, Martyna Waluś¹, Michał Dorosz¹, Michał A. Gryziński¹

¹Nuclear Facilities Operations Department, National Centre for Nuclear Research, Sołtana 7, 05-400 Otwock, Poland

Radiation-induced DNA damage response activated by neutron mixed-beam used in boron neutron capture therapy (BNCT) has not been fully established. This protocol provides a step-by-step procedure to detect radiation-induced foci (RIF) of repair proteins by immunofluorescence staining in human colon cancer cell lines after irradiation with the neutron-mixed beam.