All India Institute of Medical Sciences Jodhpur

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Obtaining Human Microglia from Adult Human Brain Tissue

Ishan Agrawal¹, Shivanjali Saxena¹, Preethika Nair¹, Deepak Jha², Sushmita Jha¹

¹Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, ²Department of Neurosurgery, All India Institute of Medical Sciences Jodhpur

This protocol is an efficient, cost effective and robust method of isolating primary microglia from live, adult, human brain tissue. Isolated primary human microglia can serve as a tool for studying cellular processes in homeostasis and disease.