Deutsches Zentrum für Herz-Kreislaufforschung (DZHK)

1 ARTICLES PUBLISHED IN JoVE

Genetics

Optimized High Quality DNA Extraction from Formalin-Fixed Paraffin-Embedded Human Atherosclerotic Lesions

Sabine Bauer^1,2,4, Jessica Pauli^1,2, Jaroslav Pelisek^1,3

¹Department for Vascular and Endovascular Surgery, Klinikum rechts der Isar, Technical University Munich, ²partner site Munich Heart Alliance, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK), ³Department of Vascular Surgery, University Hospital Zurich, ⁴Department of Experimental Cardiology, German Heart Centre, Technical University Munich

Here, we present a protocol for semi-automated DNA extraction from formalin-fixed paraffin-embedded lesions of human carotid arteries. The tissue lysis is performed without toxic xylene, which is followed by an automated DNA extraction protocol, including a second lysis step, binding of DNA to paramagnetic particles for cellulose based binding, washing steps, and DNA elution.