Naked-Eye Detection of Rare Point Mutations in DNAGayatri Udayan *1,2, Alessandra Marsella *1,2, Paola Valentini 3
1Center for Bio-Molecular Nanotechnologies, Istituto Italiano di Tecnologia (IIT), 2Department of Engineering for Innovation, University of Salento, 3Center for Human Technologies (CHT), Central RNA Laboratory, Istituto Italiano di Tecnologia (IIT)
This protocol permits the naked-eye identification of point mutated DNA in a 200-fold excess of wild type DNA molecules, by exploiting gold nanoparticles and paramagnetic microparticles.
