Université Grenoble Alpes, CEA, CNRS, IBS

2 ARTICLES PUBLISHED IN JoVE

Chemistry

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

Sofia Jaho¹, Niels Junius^1,3, Franck Borel¹, Yoann Sallaz-Damaz¹, Jean-Baptiste Salmon², Monika Budayova-Spano¹

¹Université Grenoble Alpes, CEA, CNRS, IBS, ²CNRS, Solvay LOF, UMR 5258, Université Bordeaux, ³ELVESYS SAS

This paper details the fabrication protocol of microfluidic chips developed for on-chip protein crystallization with the dialysis method and in situ X-ray diffraction experiments. The microfabrication process makes it possible to integrate a semipermeable regenerated cellulose dialysis membrane with any molecular weight cut-off, between two layers of the chip.

Biology

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Elham Vahdatahar¹, Niels Junius^1,2, Monika Budayova - Spano¹

¹CEA, CNRS, IBS, Université Grenoble Alpes, ²ELVESYS SAS

Structural studies of biomacromolecules by crystallography require high-quality crystals. Here we demonstrate a protocol that can be used by OptiCrys (a fully automated instrument developed in our lab) and/or microdialysis buttons for growing large high-quality crystals based on knowledge of the crystallization phase diagram.