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Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital

1 ARTICLES PUBLISHED IN JoVE

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Genetics

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
Sampath Kumar Loganathan 1, Ahmad Malik 1,2, Ellen Langille 1,2, Chen Luxenburg 3, Daniel Schramek 1,2
1Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics, University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent mice.

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