Hospital Universitario Virgen del Rocío/CSIC/ Universidad de Sevilla, Sevilla, Spain

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

Victor Escrich¹, Begoña Ezcurra¹, Eva Gómez-Orte¹, Cristina Romero-Aranda¹, Antonio Miranda-Vizuete², Juan Cabello¹

¹CIBIR (Center for Biomedical Research of La Rioja), La Rioja, Spain, ²IBIS (Instituto de Biomedicina de Sevilla), Hospital Universitario Virgen del Rocío/CSIC/ Universidad de Sevilla, Sevilla, Spain

Here, we present a protocol for preparing and mounting Caenorhabditis elegans embryos, recording development under a 4D microscope and tracing cell lineage.