Three-Dimensional Adipocyte Culture as a Model to Study Cachexia-Induced White Adipose Tissue RemodelingMiguel L Batista Jr. 1,2, Tova Meshulam 2,3, Kathleen Desevin 2, Nabil Rabhi 2, Stephen R. Farmer 2
1Department of Integrated Biotechnology, University of Mogi das Cruzes, 2Department of Biochemistry, Boston University School of Medicine, 3Obesity Research Center, Department of Medicine, Boston University School of Medicine
This protocol describes a three-dimensional (3D) magnetic printing culture system that permits dissection of white adipose tissue (WAT) remodeling induced by a conditioned medium from cancer cells. Using a 3D culture system of UCP1+ adipocytes that express a green fluorescent protein (GFP) allows the study of beige adipocytes contributing to adipose tissue remodeling.
