Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular KineticsAnita Reiser 1, Daniel Woschée 1, Simon Maximilian Kempe 1, Joachim Oskar Rädler 1
1Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität München, Geschwister-Scholl-Platz 1, 80539 München, Germany
We present a method for the acquisition of fluorescence reporter time courses from single cells using micropatterned arrays. The protocol describes the preparation of single-cell arrays, the setup and operation of live-cell scanning time-lapse microscopy and an open-source image analysis tool for automated preselection, visual control and tracking of cell-integrated fluorescence time courses per adhesion site.
