Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement AttributesJeremy P. Goering *1, Dona Greta Isai *1, Andras Czirok 1,2, Irfan Saadi 1
1Department of Anatomy and Cell Biology, University of Kansas Medical Center, 2Department of Biological Physics, Eotvos University
We present a protocol for isolation and culture of primary mouse embryonic palatal mesenchymal cells for time-lapse imaging of two-dimensional (2D) growth and wound-repair assays. We also provide the methodology for analysis of the time-lapse imaging data to determine cell-stream formation and directional motility.
