University of Sao Paulo, Ribeirao Preto, SP, Brazil

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

Camila Gachet-Castro^*1, Lays Adrianne Mendonça Trajano-Silva^*1, Munira Muhammad Abdel Baqui¹

¹Laboratory of Cell and Molecular Biology of Trypanosomatids, Department of Cellular and Molecular Biology and Pathogenic Bioagents, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, SP, Brazil

Here, the protocol describes how to perform double labeling immunofluorescence using primary antibodies raised in the same species to study host-pathogen interactions. Also, it can include the third antibody from a different host in this protocol. This approach can be made in any cell type and pathogens.