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Campus Universidad Autónoma de Madrid

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Cryo-EM and Single-Particle Analysis with Scipion
A. Jiménez-Moreno 1, L. del Caño 1, M. Martínez 1, E. Ramírez-Aportela 1, A. Cuervo 1, R. Melero 1, R. Sánchez-García 1, D. Strelak 1,2,3, E. Fernández-Giménez 1, F.P. de Isidro-Gómez 1, D. Herreros 1, P. Conesa 1, Y. Fonseca 1, D. Maluenda 1, J. Jiménez de la Morena 1, J.R. Macías 1, P. Losana 1, R. Marabini 1, J.M. Carazo 1, C.O.S. Sorzano 1,4
1Centro Nacional de Biotecnología, Campus Universidad Autónoma de Madrid, 2Faculty of Informatics, Masaryk University, 3Institute of Computer Science, Masaryk University, 4Campus Urbanización Montepríncipe, Universidad San Pablo CEU

Single-particle analysis in cryo-electron microscopy is one of the main techniques used to determine the structure of biological ensembles at high resolution. Scipion provides the tools to create the whole pipeline to process the information acquired by the microscope and achieve a 3D reconstruction of the biological specimen.

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