Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation BlockNatàlia de Martín Garrido 1, Kailash Ramlaul 1, Christopher H. S. Aylett 1
1Section of Structural & Synthetic Biology, Department of Infectious Diseases, Faculty of Medicine, Imperial College London, South Kensington Campus
Sample preparation for cryo-electron microscopy (cryo-EM) is a significant bottleneck in the structure determination workflow of this method. Here, we provide detailed methods for using an easy-to-use, three-dimensionally printed block for the preparation of support films to stabilize samples for transmission EM studies.