Three-Dimensional Imaging of Organoids to Study Primary Ciliogenesis During ex vivo OrganogenesisMaela Duclos 1, Claude Prigent 1,2, Roland Le Borgne 1, Vincent J. Guen 1,3
1Univ Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes), 2CRBM, CNRS, University Montpellier, 3CRCINA, INSERM, CNRS, Université d’Angers, Université de Nantes
Stem cell-derived organoids facilitate the analysis of molecular and cellular processes that regulate stem cell self-renewal and differentiation during organogenesis in mammalian tissues. Here we present a protocol for the analysis of the biology of the primary cilium in mouse mammary organoids.
