Marine Works Japan Ltd.

1 ARTICLES PUBLISHED IN JoVE

Biology

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

Masashi Yamaguchi¹, Azusa Takahashi-Nakaguchi¹, Katsuyuki Uematsu², Masaki Taguchi², Michiyo Sato-Okamoto¹, Hiroji Chibana¹

¹Medical Mycology Research Center, Chiba University, ²Marine Works Japan Ltd.

Here we show how to use the sandwich freezing device for rapid freezing of biological specimens, including bacteria, yeast, cultured cells, isolated cells, animal and human tissues, and viruses. We also show how to prepare specimens for ultrathin sectioning after rapid freezing.