National Institute of Science and Technology (INCT), Research Institute in Immunology

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Obtainment of Macrophages from Human Monocytes to Assess Leishmania braziliensis Infection Rate and Innate Host Immune Response

Icaro Bonyek-Silva^1,2, Sara Nunes^1,2, Rana Bastos¹, Reinan Lima^1,2, Leilane Barbosa^1,2, Gabriela Grimaldi¹, Vinicius Rocha⁴, Milena B. P. Soares^1,4, Patrícia S T Veras^1,3, Juliana de Menezes^1,2, Cláudia Brodskyn^1,2,3, Natalia Tavares^1,2

¹Oswaldo Cruz Foundation, Gonçalo Moniz Institute, ²Federal University of Bahia, ³National Institute of Science and Technology (INCT), Research Institute in Immunology, ⁴Senai Institute for Innovation in Advanced Health Systems, National Service for Industrial Learning, Integrated Manufacturing and Technology Campus, SENAI - CIMATEC Salvador

This protocol describes the process for obtaining human macrophages from monocytes for infection with Leishmania braziliensis. It also allows researchers to evaluate infection rate and parasite viability, ROS production by fluorescence microscopy, and the production of inflammatory mediators in culture supernatants to investigate macrophage response to infection.