Canadian Institute for Advanced Research (CIFAR)

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

Katharine M. Ng^1,2, Carolina Tropini^1,2,3

¹School of Biomedical Engineering, University of British Columbia, ²Department of Microbiology and Immunology, University of British Columbia, ³Humans and the Microbiome Program, Canadian Institute for Advanced Research (CIFAR)

This streamlined protocol details a workflow to detect and image bacteria in complex tissue samples, from fixing the tissue to staining microbes with fluorescent in situ hybridization.

Chemistry

Hydrogen Production and Utilization in a Membrane Reactor

Alexandra R. Rousseau^*1, Mia D. Stankovic^*2, Curtis P. Berlinguette^1,2,3,4

¹Department of Chemical and Biological Engineering, The University of British Columbia, ²Department of Chemistry, The University of British Columbia, ³Stewart Blusson Quantum Matter Institute, The University of British Columbia, ⁴Canadian Institute for Advanced Research (CIFAR)

Membrane reactors enable hydrogenation in ambient conditions without direct H₂ input. We can track the hydrogen production and utilization in these systems using atmospheric mass spectrometry (atm-MS) and gas chromatography mass spectrometry (GC-MS).