Women & Infants Hospital of Rhode Island and Warren Alpert Medical School of Brown University

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Adhesion of Candida parapsilosis to Bovine Serum Albumin under Fluid Shear

Sunil K. Shaw¹, Sarah J. Longley¹, Sonia S. Laforce-Nesbitt¹, Joseph M. Bliss¹

¹Women & Infants Hospital of Rhode Island and Warren Alpert Medical School of Brown University

Adhesion is an important first step in colonization and pathogenesis for Candida. Here, an in vitro assay is described to measure adhesion of C. parapsilosis isolates to immobilized proteins under fluid shear. A multichannel microfluidics device is used to compare multiple samples in parallel, followed by quantification using fluorescence imaging.