An Affordable and Efficient "Homemade" Platform for Drosophila Behavioral Studies, and an Accompanying Protocol for Larval Mitochondrial RespirometryGeovana S. Garcia 1, Murilo F. Othonicar 1, Marcos T. Oliveira 1, Carlos A. Couto-Lima 2
1Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista “Júlio de Mesquita Filho”, 2Faculdade de Ciências da Saúde, Biomedicina, Universidade do Oeste Paulista
We provide protocols for anyone with a "maker culture" mind to start building a flylab for quantitative analysis of a myriad of behavioral parameters in Drosophila melanogaster, by 3D-printing many of the necessary pieces of equipment. We also describe a high resolution respirometry protocol using larvae to combine behavioral and mitochondrial metabolism data.
