Nuclear Migration in the Drosophila OocyteMaëlys Loh 1, Antoine Guichet 1, Fred Bernard 1
1Université de Paris, CNRS, Institut Jacques Monod
In Drosophila, the oocyte nucleus undergoes microtubule-dependent migration during oogenesis. Here, we describe a protocol that was developed to follow the migration by performing live imaging on egg chambers ex-vivo. Our procedure maintains egg chambers alive for 12 h to acquire multi-position 3D time-lapse movies using spinning-disk confocal microscopy.
