Probing Metabolism and Viscosity of Cancer Cells using Fluorescence Lifetime Imaging MicroscopyLiubov Shimolina 1, Maria Lukina 1, Vladislav Shcheslavskiy 1,2, Vadim Elagin 1, Varvara Dudenkova 1, Nadezhda Ignatova 1, Marina K. Kuimova 3, Marina Shirmanova 1
1Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 2Becker & Hickl GmbH, 3Department of Chemistry, Imperial College London
Here, we demonstrate the use of fluorescence lifetime imaging microscopy (FLIM) to sequentially image cellular metabolism and plasma membrane viscosity in live cancer cell culture. Metabolic assessments are performed by detecting endogenous fluorescence. Viscosity is measured using a fluorescent molecular rotor.
