Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant MetabolomicsJuan M. Lopez 1, Vanessa Leyva 1, Helena Maruenda 1
1Departamento de Ciencias - Química, CERMN, Pontificia Universidad Católica del Perú
This paper presents the use of PSYCHE and SAPPHIRE-PSYCHE in the metabolic profiling of plants and includes detailed procedures for sample preparation and optimal Pure Shift NMR spectra recording. Examples through which the gain in resolution achieved by homonuclear decoupling allows a more comprehensive understanding of the system are discussed.
