Karabuk University

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection

Haci Mehmet Kayili¹, Bekir Salih²

¹Department of Biomedical Engineering, Karabuk University, ²Department of Chemistry, Hacettepe University

N-glycan profiling of glycoproteins is essential for discovering novel biomarkers and understanding glycan functions in cellular events. Additionally, N-glycan analysis of protein biopharmaceuticals is very important for human use. In this current article, a high-throughput strategy for identifying and quantifying N-glycan structures was presented using the HILIC-FLD-MS/MS technique.

Biochemistry

Recombinant Production of Bifidobacterial Endoglycosidases for N-glycan Release

Berfin Sucu¹, Ayşe Bayraktar^1,2, Hatice Duman¹, Ayşenur Arslan¹, Merve Kaplan¹, Melda Karyelioğlu¹, Eda Ntelitze¹, Taner Taştekin¹, Seray Yetkin¹, Melih Ertürk², Steven A. Frese^3,4, Bethany M. Henrick^4,5, Hacı Mehmet Kayili⁶, Bekir Salih⁷, Sercan Karav¹

¹Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, ²Uluova Dairy Farm, ³Department of Nutrition, University of Nevada, ⁴Department of Food Science and Technology, University of Nebraska, ⁵Evolve BioSystems Inc., ⁶Department of Biomedical Engineering, Karabuk University, ⁷Department of Chemistry, Hacettepe University

Bifidobacteria possess a unique genomic capability for N-glycan cleavage. Recombinantly producing these enzymes would be a promising novel tool to release bioactive N-glycans from glycoprotein-rich substrates such as colostrum.