Çanakkale Onsekiz Mart University

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Recombinant Production of Bifidobacterial Endoglycosidases for N-glycan Release

Berfin Sucu¹, Ayşe Bayraktar^1,2, Hatice Duman¹, Ayşenur Arslan¹, Merve Kaplan¹, Melda Karyelioğlu¹, Eda Ntelitze¹, Taner Taştekin¹, Seray Yetkin¹, Melih Ertürk², Steven A. Frese^3,4, Bethany M. Henrick^4,5, Hacı Mehmet Kayili⁶, Bekir Salih⁷, Sercan Karav¹

¹Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, ²Uluova Dairy Farm, ³Department of Nutrition, University of Nevada, ⁴Department of Food Science and Technology, University of Nebraska, ⁵Evolve BioSystems Inc., ⁶Department of Biomedical Engineering, Karabuk University, ⁷Department of Chemistry, Hacettepe University

Bifidobacteria possess a unique genomic capability for N-glycan cleavage. Recombinantly producing these enzymes would be a promising novel tool to release bioactive N-glycans from glycoprotein-rich substrates such as colostrum.

Biochemistry

Determining Total Protein and Bioactive Protein Concentrations in Bovine Colostrum

Ayşenur Arslan¹, Hatice Duman¹, Merve Kaplan¹, Hasan Uzkuç¹, Ayşe Bayraktar^1,2, Melih Ertürk², Merve Alkan^1,2, Steven A. Frese^3,4, Rebbeca M. Duar⁵, Bethany M. Henrick^4,5, Sercan Karav¹

¹Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, ²Uluova Dairy Farm, ³Department of Nutrition, University of Nevada, Reno, ⁴Department of Food Science and Technology, University of Nebraska, Lincoln, ⁵Evolve BioSystems Inc.

Bovine colostrum is both a primary source of nutrients and immunological support for the newborn calf. The understanding of the level of therapeutic proteins (lactoferrin and IgG) is important for the bovine colostrum dosing and standardization for human consumption.