Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial CompoundsJosselin Rigaill *1,2, Estelle Audoux *1, Killian Rodriguez 1, Aurélien Peyron 1, Philippe Berthelot 1,3, Jérôme Josse 4,5, Frédéric Laurent 4,5, Robin Caire 1, Paul O. Verhoeven 1,2
1CIRI, Centre International de Recherche en Infectiologie, GIMAP team, University of Lyon, INSERM U1111, CNRS, UMR5308, ENS Lyon, UCBL1, University of St-Etienne, France, 2Department of Infectious Agents and Hygiene, University Hospital of St-Etienne, St-Etienne, France, 3Department of Infectious Diseases, University Hospital of St-Etienne, St-Etienne, France, 4CIRI, Centre International de Recherche en Infectiologie, Staphylococcal Pathogenesis team, University of Lyon, INSERM U1111, CNRS UMR5308, ENS Lyon, UCBL1, University of Lyon, Lyon, France, 5Department of Bacteriology, Institute for Infectious Agents, Hospices Civils de Lyon, Lyon, France
This protocol aims to describe how to study the extent of Staphylococcus aureus internalization and its ability to survive inside the human host cell, as well as the intracellular efficacy of antimicrobial compounds.
