Live-imaging of Mitochondrial System in Cultured AstrocytesJeanne Espourteille 1, Valentin Zufferey 1, Jean-Honoré Laurent 1, Kevin Richetin 1
1Department of Psychiatry, Center for Psychiatric Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne
This article describes the method for mitochondrial time-lapse imaging of astrocyte cultures equipped with MitoTimer biosensor and the resulting multiparametric analysis of mitochondrial dynamics, mobility, morphology, biogenesis, redox state, and turnover.