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National Cerebral and Cardiovascular Center Research Institute

2 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
Yuji Nashimoto 1, Yukako Teraoka 1, Ramin Banan Sadeghian 1, Akiko Nakamasu 2, Yuichiro Arima 3, Sanshiro Hanada 3, Hidetoshi Kotera 1, Koichi Nishiyama 3, Takashi Miura 2, Ryuji Yokokawa 1
1Department of Micro Engineering, Kyoto University, 2Graduate School of Medical Sciences, Kyushu University, 3International Research Center for Medical Sciences (IRCMS), Kumamoto University

The protocol describes how to engineer a perfusable vascular network in a spheroid. The spheroid's surrounding microenvironment is devised to induce angiogenesis and connect the spheroid to the microchannels in a microfluidic device. The method allows the perfusion of the spheroid, which is a long-awaited technique in three-dimensional cultures.

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Medicine

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels
Daiki Seya 1, Yuqing Xu 2,3, Toshifumi Mukunoki 4, Kenichi Tsujita 3, Osamu Nakagawa 1, Yuichiro Arima 1,2,3,4
1Department of Molecular Physiology, National Cerebral and Cardiovascular Center Research Institute, 2International Research Center for Medical Sciences (IRCMS), Kumamoto University, 3Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, 4X-Earth Center, Faculty of Advanced Science and Technology, Kumamoto University

Here, we describe a visualization and quantification method for murine hind-limb vessels using micro-X-ray computed tomography.

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