College of Medicine-Phoenix, University of Arizona

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

A Novel Tenorrhaphy Suture Technique with Tissue Engineered Collagen Graft to Repair Large Tendon Defects

Prasad Sawadkar^1,3, Jason Wong², Vivek Mudera¹

¹Division of Surgery and Interventional Science, University College London, ²Blond McIndoe Laboratories, Division of Cell Matrix and Regenerative Medicine, MAHSC, University of Manchester, ³Department of Surgical Research, Northwick Park Institute for Medical Research, Northwick Park Hospital

In this paper, we present an in vitro and in situ protocol to repair a tendon gap of up to 1.5 cm by filling it with engineered collagen graft. This was performed by developing a modified suture technique to take the mechanical load until the graft matures into the host tissue.

Developmental Biology

Pan-myeloid Differentiation of Human Cord Blood Derived CD34⁺ Hematopoietic Stem and Progenitor Cells

Aditi Bapat¹, Nakia Keita¹, Shalini Sharma¹

¹Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona

Here, we present a protocol for immunophenotypic characterization and cytokine induced differentiation of cord blood derived CD34⁺ hematopoietic stem and progenitor cells to the four myeloid lineages. The applications of this protocol include investigations on the effect of myeloid disease mutations or small molecules on myeloid differentiation of the CD34⁺ cells.

JoVE Journal

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Jason Wong¹, William Martelly¹, Shalini Sharma¹

¹Department of Basic Medical Sciences, College of Medicine-Phoenix, University of Arizona

This protocol describes a minigene reporter assay to monitor the impact of 5´-splice site mutations on splicing and develops suppressor U1 snRNA for the rescue of mutation-induced splicing inhibition. The reporter and suppressor U1 snRNA constructs are expressed in HeLa cells, and splicing is analyzed by primer extension or RT-PCR.