Genethon, 91000, Evry, France

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Martina Marinello^*1,2, Jérémie Cosette^*1, Caroline Bogni^1,2, Jérôme Denard^1,2, Daniel Stockholm^*1,3, Ana Buj-Bello^*1,2

¹Genethon, 91000, Evry, France, ²Université Paris-Saclay, Univ Evry, Inserm, Genethon, Integrare research unit UMR_S951, 91000, Evry, France, ³Ecole Pratique des Hautes Etudes, PSL Research University, 75014, Paris, France

This protocol describes a method for morphometric analysis of neuromuscular junctions by combined confocal and STED microscopy that is used to quantify pathological changes in mouse models of SMA and ColQ-related CMS.