Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term CryopreservationLeniza Pola-Silva 1,2, Fabio Xerfan Nahas 3, Flavia Nascimento 2, Tatiana Rabelo Santos 2, Andrea Moraes Malinverni 1, Adelson Alves 2, Lydia Masako Ferreira 3, Maria Isabel Melaragno 1
1Genetics Division, Universidade Federal de São Paulo (UNIFESP), 2TechLife Biotech, São Lucas Cell Therapy Group, 3Division of Plastic Surgery, Universidade Federal de São Paulo (UNIFESP)
The present protocol describes an improved methodology for ADSC isolation resulting in a tremendous cellular yield with time gain compared to the literature. This study also provides a straightforward method for obtaining a relatively large number of viable cells after long-term cryopreservation.
