The Hebrew University-Hadassah Medical School

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

Balakrishnan Solaimuthu¹, Arata Hayashi¹, Anees Khatib¹, Yoav D. Shaul¹

¹Department of Biochemistry and Molecular Biology, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School

Here, we describe a noninvasive monitoring method involving luciferase and green fluorescent protein expression in various breast cancer cell lines. This protocol provides a technique to monitor tumor formation and metastatic colonization in real time in mice.

Biology

Simple Continuous Glucose Monitoring in Freely Moving Mice

Doron Kleiman¹, Mika Littor^1,2, Mahmoud Nawas³, Rachel Ben-Haroush Schyr¹, Danny Ben-Zvi¹

¹Department of Developmental Biology and Cancer Research, Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, ²Department of Military Medicine and “Tzameret”, Faculty of Medicine, Hebrew University of Jerusalem, and Medical Corps, Israel Defense Forces, ³Department of Surgery, Hadassah Medical Center-Ein Kerem Campus

Here, we describe a simple method to implant a commercial continuous glucose monitor designed for patients onto mice and provide the scripts to analyze the results.