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Universidad Católica del Norte

3 ARTICLES PUBLISHED IN JoVE

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Behavior

An Inescapable Cat Odor Exposure Protocol for Studying Innate and Contextual Threat Conditioning in Rats
María Rodríguez 1,2, Marco Contreras 3, Marcos Domic-Siede 4, Francisco Ceric 5, Fernando Torrealba 1
1Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, 2Instituto de Ciencias Sociales, Universidad de O'Higgins, 3Department of Psychology, University of Arizona, Tucson, 4Escuela de Psicología, Universidad Católica del Norte, 5Laboratorio de Neurociencia Afectiva, Facultad de Psicología, Universidad del Desarrollo

Cat skin/fur odor exposure provides a reliable method to investigate neural circuits and mechanisms of defensive responses in rodents and may offer insight into mechanisms that mediate fear in humans. Here, we describe a protocol for investigating the role of the interoceptive cortex in threat responses in rats.

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Neuroscience

A Visuospatial Planning Task Coupled with Eye-Tracker and Electroencephalogram Systems
Marcos Domic-Siede 1, Martín Irani 2, Joaquín Valdés 3, María Rodríguez 4,5, Brice Follet 3,6, Marcela Perrone-Bertolotti 7,8, Tomás Ossandón 3
1Laboratorio de Neurociencia Cognitiva, Escuela de Psicología, Universidad Católica del Norte, 2Department of Psychology, University of Illinois Urbana-Champaign, 3Neurodynamics of Cognition Laboratory, Departamento de Psiquiatría, Pontificia Universidad Católica de Chile, 4Instituto de Ciencias de la Salud, Universidad de O’Higgins, 5Instituto de Ciencias de la Ingeniería, Universidad de O’Higgins, 6Department of Psychiatry, University Hospital of Saint-Etienne, 7Univ. Grenoble Alpes, Univ. Savoie Mont Blanc, CNRS, LPNC, 8Institut Universitaire de France (IUF)

The study of cognitive planning combining EEG and eye-tracking systems provides a multimodal approach to investigate the neural mechanisms that mediate cognitive control and goal-directed behavior in humans. Here, we describe a protocol for investigating the role of brain oscillations and eye movements in planning performance.

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Neuroscience

Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor
Jeanette Wihan 1, Isabell Karnatz 1, Isabelle Sébastien 1, Ralf Kettenhofen 1, Benjamin Schmid 2, Christian Clausen 2, Benjamin Fischer 1, Rachel Steeg 3, Heiko Zimmermann 1,4,5,6, Julia C. Neubauer 1,4
1Fraunhofer Project Center for Stem Cell Process Engineering, Fraunhofer Institute for Biomedical Engineering IBMT, 2Bioneer A/S, 3Fraunhofer UK Research Ltd, Technology and Innovation Centre, 4Fraunhofer Institute for Biomedical Engineering IBMT, 5Department of Molecular and Cellular Biotechnology, Saarland University, 6Facultad de Ciencias del Mar, Universidad Católica del Norte

This article describes a protocol for the generation of human induced pluripotent stem cell-derived neurons in a benchtop 3D suspension bioreactor.

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