Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression SystemJames B. Pace 1, Nian N. Huang 1, Jacqueline P. Séguin 1, Candi Esquina 1, Erin Olin 1, Guangyu Zhu 1, Grant Carr 1
1Discovery Biology, Curia Global Inc.
We provide scalable protocols covering construct design, transient transfection, and expression and purification of full-length human huntingtin protein variants in HEK293 cells.
